Gene construction kit error 1997/9/2023 Keane, Julian Parkhill, "Roary: Rapid large-scale prokaryote pan genome analysis", Bioinformatics, 2015 31(22):3691-3693 doi:10.1093/bioinformatics/btv421 Step by step tutorial Holden, Maria Fookes, Daniel Falush, Jacqueline A. Citation and further details of the methodĪndrew J. Roary is not intended for meta-genomics or for comparing extremely diverse sets of genomes. To perform this analysis using existing methods would take weeks and hundreds of GB of RAM. ![]() 128 samples can be analysed in under 1 hour using 1 GB of RAM and a single processor. ![]() ![]() Using a standard desktop PC, it can analyse datasets with thousands of samples, something which is computationally infeasible with existing methods, without compromising the quality of the results. Roary is a high speed stand alone pan genome pipeline, which takes annotated assemblies in GFF3 format (produced by Prokka (Seemann, 2014)) and calculates the pan genome. By Andrew Page based on version 3.11.2 (2)
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